Characterization of protein phosphorylation reaction in AH-66 hepatoma ascites cells
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چکیده
منابع مشابه
Characterization of messenger RNA for fructose 1,6-bisphosphate aldolase A isozyme of rat ascites hepatoma AH 7974 cells.
The messenger activity for fructose 1,6-bisphosphate aldolase (EC4.1.2.13) (aldolase) A isozyme has been characterized in the polysome- or the messenger RNA-directed, protein-synthesizing system using the pH 5 fraction of rat liver or wheat germ extracts, respectively. The subunit of aldolase A synthesized in vitro was detected by immunoprecipitation with anti-aldolase A antibody raised in chic...
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A study of the enzymes of the glycogen pathway in Novikoff ascites hepatoma shows that glycogen synthetase has the lowest activity and that the tumour contains no high-K(m) soluble glucokinase. However, incubation of tumour cells with metabolizable sugars in vitro, or intraperitoneal administration of glucose into the tumour-bearing rat, results in glycogen accumulation by the tumour cells. Gly...
متن کاملPasteur Effect in Novikoff Ascites-hepatoma Cells.
Inhibition of fermentation or of glucose uptake in the presence ofoxygen has been observed in many forms of life and is generally referred to as the 'Pasteur effect'. In most tissues, oxidative inhibition of fermentation (ethanol or lactate production) is reflected by a parallel decrease in glucose consumption. Since certain tissues utilize lactate significantly, estimation of the Pasteur effec...
متن کاملproten tyrosine phosphatase activity in rat ascites hepatoma cells
protein tyrosine phosphatases (ptpases) regulate tyrosine phosphorylation of target proteins involved in several aspects of cellular functions. enzyme activities of the ptpases in cytosolic and particulate fractions of rat ascites hepatoma cell lines were determined and compared with those of normal rat liver. our present data revealed that although there was no neoplatic-specific alteration of...
متن کاملMarked hyperlipidaemia in rats bearing the Yoshida AH-130 ascites hepatoma.
Malignant tumour burden is a pathological s ta te associated with a considerable demand for nutrients by the growing tumour [l]. Although a great deal of work has been carried out, there are contradictory results about the effects of tumour burden on lipid metabolism both in experimental animals and man. Lipid oxidation has been reported either increased [2], unchanged [3] or decreased [4]. In ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1982
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)53939-7